Several established optical imaging approaches have been applied, usually in isolation, to preclinical studies; however, as illustrated here, truly useful in-vivo imaging requires a simultaneous combination of imaging modalities to examine dynamic characteristics of cells and tissues. MILabs developed a new multimode optical imaging system designed to be application-versatile, yielding high sensitivity and specificity. Most importantly, the system enables effortless translation from bench to clinic through a one-pass, in-line scalable multimodality optical imaging platform.
Start with an improved IVIS* alternative…
With the ability to visualize bioluminescence, fluorescence, and Cherenkov activity, and a wide choice of optical probes combined with numerous filters for selective imaging, optical methods have many flexible and powerful modes for providing both molecular and functional contrast. For instance, with the new MILabs’ Duet Optical Imaging module, which is, in essence, a state-of-the-art IVIS* system, up to ten mice can be imaged simultaneously.
However, no matter how sophisticated the design, with deep-cooling and back-illuminated detection, the effect of optical probe depth on the detected signal remains dramatic as illustrated. For further reading: U-SPECT-BioFluo
…Upgrade to the full benefits of deep-tissue imaging and optical tomography
To overcome the light attenuation and scatter obstacles to optical imaging of deep tissue, MILabs enables the mounting of the Duet Optical Module in-line with a top-level CT system. This low-dose, high soft-tissue contrast CT system allows highly detailed assigning of optical properties, including light absorption and scattering, to various tissues and organs according to high precision anatomic segmentation.
Light absorption is derived from measured CT attenuation, and scattering maps are stored in the system based on hundreds of in-vivo animal measurements. This in-line configuration is exciting because one can obtain complementary data from both contrast mechanisms within the same device, resulting in both detailed anatomical information and 3D optical tomographic images. For further reading: Improved Biodistributions Using Hybrid Optical/CT
The whole picture just got clearer…
MILabs in-line hybrid OI/CT system is the only device in the industry that includes an autonomous state-of-the-art CT. The system allows for cardiac- and respiratory-gated imaging and features sensor-free respiratory gating for imaging up to four mice simultaneously. Acquisitions are fast, enabling DCE-CT and dynamic multi-frame imaging.
An on-screen radiation dose estimator and dual-energy acquisitions add to this system’s outstanding in-vivo CT capabilities, whether used for CT-guided optical tomography using anatomical priors or for sharp, detailed in-vivo CT. For further reading: Diagnostic and Molecular CT
…and the data more quantifiable.
For proper quantitative fluorescence reconstructions, knowledge of the highly heterogeneous scattering and absorption in different tissues is required. The following pictures of a quantitative biodistribution analysis illustrate that 3D reconstruction with a heterogeneous absorption and scattering map based on CT-prior information rather than by a homogeneous absorption reconstruction without the use of CT-priors is essential to avoid a systematic underestimation of fluorescence in strongly absorbing organs, leading to significant errors as illustrated for the heart, liver, and kidney images. For further reading: Sensitivity & Accuracy of 3D Optical Imaging
Perform true in-vivo 3D/4D Optical imaging research
Accurate spatial and temporal resolution images are required to determine the biodistribution of nanomedicine particles under development by Cristal Therapeutics. This company uses a pioneering approach to transform the efficacy and side-effect profiles of therapeutic candidates by creating tailor-made nanoparticles. They are using a rational design to optimize efficacy and reduce toxicity.
As illustrated by the fluorescence tomography CT images (FLT/CT), their Cripec-Cy7 particles show the desired biodistribution with long distribution times, high uptake in the tumor and good clearance from other tissues and organs. In addition, in-vivo optical tomography quantitation shows excellent correlation with ex-vivo studies. For further reading: Nanoparticles with Neglectable Protein Corona
Bring your research to a new level with in-line Optical and Nuclear imaging
Optical and nuclear methods have a symbiotic relationship that is being explored by an increasing number of customers worldwide. PET, SPECT, and CT-guided Optical Tomography offer sensitivity and resolution unparalleled by other in-vivo biomedical imaging methods. By combining these optical modalities on a single-pass, in-line platform, we believe that MILabs offers its customers the ability to translate experimental findings from cells and tissues on the bench to the complex multicellular milieu in living organisms. As illustrated in a video, the MILabs platform can provide a nearly seamless translation from bench to clinical-ready applications. For further viewing: MILabs in 35 Seconds